Prolonged incubation with NdeI may lead to removal of additional nucleotides.Įfficient cleavage requires at least two copies of the SgrAI recognition sequence.Ĭ C T N N N N N A G G G G A N N N N N T C C Sticky ends from different BlpI sites may not be compatible.Īfter cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. Sticky ends from different Esp3I sites may not be compatible.
0 Comments
Leave a Reply. |